Enzymes

T7 RNA Polymerase

This product is a recombinant source of T7 RNA polymerase from Escherichia coli. It is a DNA-dependent RNA polymerase that specifically recognizes the T7 promoter and synthesizes RNA chains complementary to the downstream template strand. It uses single-stranded or double-stranded DNA templates containing the T7 promoter sequence and NTPs as substrates.

RNase inhibitor,Murine

This product is a recombinant mouse RNase inhibitor expressed and purified in E. coli. It can bind to RNase A, RNase B, or RNase C in a non-competitive manner in a 1:1 ratio, thereby inhibiting the activity of these three enzymes and protecting RNA from degradation. The inhibitor is compatible with various commercially available Reverse Transcriptase and DNA Polymerase enzymes. Mouse RNase inhibitor has higher antioxidant activity due to the absence of two cysteine residues in human proteins that are highly sensitive to oxidation, making it more suitable for experiments sensitive to high DTT levels, such as qPCR.

DNase I

Dnase I is a type of enzyme that can cleave DNA at phosphodiester bonds, generating DNA fragments with a 5' phosphate group and a 3' hydroxyl group. It can act on both single-stranded and double-stranded DNA, as well as chromatin DNA. The activity of DNase I depends on the presence of Ca2+ and can be activated by Mg2+ or Mn2+. In the presence of Mg2+, it can randomly recognize and cleave DNA at any site. In the presence of Mn2+, it can recognize and cleave DNA at nearly identical sites on both strands, producing DNA fragments with blunt ends or with one or two nucleotides protruding.

Inorganic Pyrophosphatase

This product is a recombinant Inorganic Pyrophosphatase derived from E. coli, which can catalyze the hydrolysis of inorganic pyrophosphate into orthophosphate. During transcription, the accumulation of inorganic pyrophosphate generated in the reaction can inhibit the production of transcription products. However, the inorganic pyrophosphatase can hydrolyze the pyrophosphate generated during the reaction, preventing the reaction from being inhibited and increasing the yield of products.

BspQ I

This product is a recombinant protein encoded by the BspQ I gene in spherical spore Bacillus subtilis expressed in Escherichia coli. It is a type IIS restriction endonuclease primarily used for cutting plasmids to prepare linear DNA fragments with poly (A/T/G/C) ends, as well as cutting DNA to obtain specific sticky ends. Its recognition sequence and cleavage site are:5'......GCTCTTC(N)......3'3'......CGAGAAG(NNNN)......5'