CRISPR-based gene editing technology enables efficient and convenient gene knockout, gene insertion, base editing, and is widely used in protein labeling, gene therapy, molecular breeding, disease model construction, and other fields. In early animal gene editing, AAV and lentivirus were used to deliver CRISPR systems, which were costly and raised safety concerns. Long-term expression of nucleases such as Cas9 may also lead to more off-target edits. With the maturity of mRNA technology, LNP-encapsulated mRNA and sgRNA targeting Cas9 can achieve targeted and efficient gene editing. Due to the transient expression of mRNA, the possibility of off-target editing can be reduced. For crop breeding, mRNA technology can achieve non-transgenic gene editing.
We offer a complete solution from gene editing scheme design to final outcome evaluation, enabling customers to easily achieve various gene editing tasks including knockout, knock-in, and base editing. This solution caters to the diverse mRNA gene editing tool requirements across different research fields, empowering customers to generate high-level scientific research outcomes efficiently.