Protein and enzyme related solutions

EnoBio has an experienced team dedicated to the development of recombinant proteins and enzymes, consisting of technical experts with over 10 years of experience in protein expression and purification/enzyme development. We have established an efficient platform for recombinant protein expression and purification, encompassing various expression systems such as prokaryotic, yeast, baculovirus-insect cell, and mammalian cell expression systems, to comprehensively meet diverse research needs of our clients.

EnoBio offers one-stop services from sequence (codon) optimization, gene synthesis, vector construction to protein expression and purification. We employ a variety of mature protein purification techniques including affinity chromatography, ion exchange (both anion and cation exchange), hydrophobic interaction chromatography, composite mode chromatography, and molecular sieving, allowing clients to choose different expression systems, expression/purification tags, etc., according to their requirements. We are committed to providing personalized, high-quality, and cost-effective recombinant protein/enzyme solutions to our clients.

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Development process:

Experimental protocol design

Retrieval of target protein information and experimental protocol design

Gene synthesis

Codon optimization

Sequence optimization

Gene synthesis

Vector construction

Plasmid construction for different protein expression systems

Prokaryotic expression system

Yeast expression system

Baculovirus-insect cell expression system

Mammalian cell expression system

IProtein expression and purification, testing

Transfection of expression vectors into host cells

Small-scale expression testing, optimization of expression conditions, analysis of expression results

Purification of target protein, and analysis of purified product

Process optimization and scale-up production

Optimization of large-scale expression of recombinant proteins

Optimization of large-scale purification process for recombinant proteins