DNase I
Product Type
key raw material for mRNA synthesis
Category
enzyme
Art.No
EM4003
Description
Dnase I is a type of enzyme that can cleave DNA at phosphodiester bonds, generating DNA fragments with a 5' phosphate group and a 3' hydroxyl group. It can act on both single-stranded and double-stranded DNA, as well as chromatin DNA. The activity of DNase I depends on the presence of Ca2+ and can be activated by Mg2+ or Mn2+. In the presence of Mg2+, it can randomly recognize and cleave DNA at any site. In the presence of Mn2+, it can recognize and cleave DNA at nearly identical sites on both strands, producing DNA fragments with blunt ends or with one or two nucleotides protruding.Product Information
| Product Type | key raw material for mRNA synthesis |
|---|---|
| Category | enzyme |
| Art.No | EM4003 |
| Scale | ① 500U② 2000U③ 10000U |
| Storage buffer | 10 mM Tris-HCl(pH 7.6,25℃),2 mM CaCl2,50%(v/v)glycerol |
| Storage temperature | -30℃~-15℃ |
| Optimum reaction temperature | 37℃ |
| Transportation | <0°C transportation |
| Active unit | The reaction was carried out at 37 °C for 10 min, and the amount of enzyme required to be able to completely degrade 1 μg of pUC19 plasmid DNA was defined as 1 activity unit (U). |
| Concentration | 1 U/μl |
Note
1. The optimum pH for this product is 7.0-8.0.
2. Place the enzyme on ice and store at -20 ℃ after use.
3. Common reagents such as platinum sub-chelators, SDS, DTT, mercaptoheptanol, etc. may inhibit the activity of this product.
4. RNAase inhibitor (EK2002) can be added during the reaction to prevent RNAase contamination, and the recommended concentration is 1-2 U/μl.
Product manual
DNase I-product manual
COA
DNase I-CoA