BspQ I
Product Type
key raw material for mRNA synthesis
Category
enzyme
Art.No
EM4005
Description
This product is a recombinant protein encoded by the BspQ I gene in spherical spore Bacillus subtilis expressed in Escherichia coli. It is a type IIS restriction endonuclease primarily used for cutting plasmids to prepare linear DNA fragments with poly (A/T/G/C) ends, as well as cutting DNA to obtain specific sticky ends. Its recognition sequence and cleavage site are:5'......GCTCTTC(N)......3'3'......CGAGAAG(NNNN)......5'Product Information
| Product Type | key raw material for mRNA synthesis |
|---|---|
| Category | enzyme |
| Art.No | EM4005 |
| Scale | ① 500U② 1000U③ 10000U④ 50000U⑤ 400000 U |
| Storage buffer | 50 mM Tris-HCl(25℃,pH 7.0),500 mM KCl,0.1mM EDTA,1.0 mM DTT,500μg/mL OsrHSA,50% Glycerol |
| Storage temperature | -20℃ |
| Optimum reaction temperature | 50℃ |
| Transportation | Dry ice |
| Active unit | The amount of enzyme required to digest 1 μg of λDNA in 1 h under 50 °C reaction conditions is defined as 1 activity unit (U) |
| Concentration | 10 U/μl |
Note
1. The volume of restriction endonuclease added should not exceed 1/10 of the reaction volume;
2. Asterisk activity may occur when the concentration of oil in the reaction system is high than 5%.
Product manual
BspQ I-product manual
COA
BspQ I-COA