RNase inhibitor,Murine
Product Type
key raw material for mRNA synthesis
Category
enzyme
Art.No
EM4002
Description
This product is a recombinant mouse RNase inhibitor expressed and purified in E. coli. It can bind to RNase A, RNase B, or RNase C in a non-competitive manner in a 1:1 ratio, thereby inhibiting the activity of these three enzymes and protecting RNA from degradation. The inhibitor is compatible with various commercially available Reverse Transcriptase and DNA Polymerase enzymes. Mouse RNase inhibitor has higher antioxidant activity due to the absence of two cysteine residues in human proteins that are highly sensitive to oxidation, making it more suitable for experiments sensitive to high DTT levels, such as qPCR.Product Information
| Product Type | key raw material for mRNA synthesis |
|---|---|
| Category | enzyme |
| Art.No | EM4002 |
| Scale | ① 2000U② 10000U③ 50000U |
| Storage buffer | 20mM HEPES-NaOH (pH7.5), 50 mM NaCl,8 mM DTT, 50% (v/v) glycerol |
| Storage temperature | -30℃~ -15℃ |
| Optimum reaction temperature | 25~55℃ |
| Transportation | <0°C transportation |
| Active unit | The amount of enzyme required to inhibit 50% of 5 ng of RNase A activity was defined as 1 unit of activity.RNase A activity was quantified by inhibiting the hydrolysis of Cyclic2',3'-CMP to produce 3'-CMP |
| Concentration | 40 U/μl |
Note
1. It is recommended that the pH range of the system be between 7.0 and 8.0 to maximize the activity of the product.
2. This product does not inhibit RNase H activity.3. Avoid repeated freezing and thawing, foaming, vigorous stirring or vortexing.4. No less than 1 mM DTT or equivalent reducing agent is required to maintain the activity of this product.
Product manual
RNase inhibitor,Murine-product manual