Inorganic Pyrophosphatase
Product Type
key raw material for mRNA synthesis
Category
enzyme
Art.No
EM4004
Description
This product is a recombinant Inorganic Pyrophosphatase derived from E. coli, which can catalyze the hydrolysis of inorganic pyrophosphate into orthophosphate. During transcription, the accumulation of inorganic pyrophosphate generated in the reaction can inhibit the production of transcription products. However, the inorganic pyrophosphatase can hydrolyze the pyrophosphate generated during the reaction, preventing the reaction from being inhibited and increasing the yield of products.Product Information
| Product Type | key raw material for mRNA synthesis |
|---|---|
| Category | enzyme |
| Art.No | EM4004 |
| Scale | ① 100U② 500U③ 2000U④ 10000U⑤ 50000 U |
| Storage buffer | 20 mM Tris-HCl(pH 8.0),100mM KCl,0.1mM EDTA,1.0 mM DTT,50% Glycerol |
| Storage temperature | -20℃ |
| Optimum reaction temperature | 25℃(Active at 16-37 ℃) |
| Transportation | Dry ice |
| Active unit | Under standard reaction conditions, the amount of enzyme required to catalyze the hydrolysis of PPi to produce 1 μmol of Pi per minute is 1 active unit. |
| Concentration | 0.1 U/μl |
Note
1. The enzyme is active in a wide range of reaction solutions and can usually be added directly to the desired reaction.
2. The enzyme is inactivated at 65°C for 10 min.
Product manual
Inorganic Pyrophosphatase--product manual