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eGFP mRNA(N1-Me-Pseudo UTP)

The eGFP protein, short for enhanced green fluorescent protein, is a commonly used reporter molecule. When stimulated by light, it emits a strong and bright green fluorescence with maximum excitation/emission wavelengths of 488 nm/509 nm. By transfecting eGFP mRNA into cells, the cells can express the eGFP protein, allowing for the study of transfection and expression within the cells.

eGFP mRNA(5-Methoxy UTP)

The eGFP protein, short for enhanced green fluorescent protein, is a commonly used reporter molecule. When stimulated by light, it emits a strong and bright green fluorescence with maximum excitation/emission wavelengths of 488 nm/509 nm. By transfecting eGFP mRNA into cells, the cells can express the eGFP protein, allowing for the study of transfection and expression within the cells.

d2eGFP mRNA(N1-Me-Pseudo UTP)

d2eGFP is a modified version of EGFP that enhances protein degradation and reduces half-life without reducing expression intensity. The d2eGFP protein can be used to precisely measure mRNA transcription and gene expression regulation. d2eGFP-mRNA has been generated through in vitro transcription and is already capped at the 5' end and poly A tailed at the 3' end.

d2eGFP mRNA(5-Methoxy UTP)

d2eGFP is a modified version of EGFP that enhances protein degradation and reduces half-life without reducing expression intensity. The d2eGFP protein can be used to precisely measure mRNA transcription and gene expression regulation. d2eGFP-mRNA has been generated through in vitro transcription and is already capped at the 5' end and poly A tailed at the 3' end.

Firefly Luciferase mRNA(N1-Me-Pseudo UTP)

Firefly Luciferase enzyme was initially isolated from the firefly Photinus pyralis and is a commonly used bioluminescent reporter molecule. It is frequently employed in research for gene regulation, cell viability assays, ATP level analysis, and in vivo imaging. After transfecting cells with Firefly Luciferase mRNA, the cells are able to express the Firefly Luciferase protein.

Firefly Luciferase mRNA(5-Methoxy UTP)

Firefly Luciferase enzyme was initially isolated from the firefly Photinus pyralis and is a commonly used bioluminescent reporter molecule. It is frequently employed in research for gene regulation, cell viability assays, ATP level analysis, and in vivo imaging. After transfecting cells with Firefly Luciferase mRNA, the cells are able to express the Firefly Luciferase protein.

EPO mRNA(N1-Me-Pseudo UTP)

The EPO mRNA codes for a hormone called erythropoietin, which controls red blood cell production in mammals. It is mainly produced in the kidneys, with a small amount produced in the liver. Erythropoietin also plays a role in healing brain injuries in wounds. Transfection of EPO mRNA in the body leads to a significant increase in reticulocyte count and hematocrit. This type of protein is also commonly used as an indicator protein to study the efficiency of cellular translation of engulfing proteins.

EPO mRNA(5-Methoxy UTP)

The EPO mRNA codes for a hormone called erythropoietin, which controls red blood cell production in mammals. It is mainly produced in the kidneys, with a small amount produced in the liver. Erythropoietin also plays a role in healing brain injuries in wounds. Transfection of EPO mRNA in the body leads to a significant increase in reticulocyte count and hematocrit. This type of protein is also commonly used as an indicator protein to study the efficiency of cellular translation of engulfing proteins.

GM-CSF mRNA(N1-Me-Pseudo UTP), Mouse

GM-CSF, also known as granulocyte-macrophage colony-stimulating factor, is a protein in mice that stimulates the formation of granulocytes and macrophages from bone marrow precursor cells. It has various physiological functions, primarily promoting the generation, differentiation, activation, and survival of these cells. Additionally, it plays a critical role as a homeostatic factor in the alveoli of the lungs, aiding in the development and long-term maintenance of alveolar macrophages. This mRNA is capped with Cap1 structure and N1-Me-Pseudo UTP modification. The UTR and poly A regions have been optimized with proprietary intellectual property rights to significantly enhance mRNA translation and expression for reduced immunogenicity and cellular toxicity.

GM-CSF mRNA(5-Methoxy UTP), Mouse

GM-CSF, Mouse mRNA expresses GM-CSF protein in mice. GM-CSF is a hematopoietic growth factor that stimulates the formation of granulocytes and macrophages from bone marrow progenitor cells. It has a wide range of physiological functions, mainly promoting the generation, differentiation, activation, and survival of granulocytes and macrophages. It is also a key homeostatic factor in the alveoli of the lungs, involved in the development and long-term maintenance of alveolar macrophages. The Enno spot-type GM-CSF mRNA (5-Methoxy UTP), Mouse uses Cap1 structure and 5-Methoxy UTP modification. The UTR and poly A regions have been optimized with proprietary intellectual property rights to significantly enhance mRNA translation and expression for reduced immunogenicity and cellular toxicity.

GM-CSF mRNA(N1-Me-Pseudo UTP), Human

GM-CSF, also known as granulocyte-macrophage colony-stimulating factor, is a protein in mice that stimulates the formation of granulocytes and macrophages from bone marrow precursor cells. It has various physiological functions, primarily promoting the generation, differentiation, activation, and survival of these cells. Additionally, it plays a critical role as a homeostatic factor in the alveoli of the lungs, aiding in the development and long-term maintenance of alveolar macrophages. This mRNA is capped with Cap1 structure and N1-Me-Pseudo UTP modification. The UTR and poly A regions have been optimized with proprietary intellectual property rights to significantly enhance mRNA translation and expression for reduced immunogenicity and cellular toxicity.

GM-CSF mRNA(5-Methoxy UTP), Human

GM-CSF, or granulocyte-macrophage colony-stimulating factor, is a protein that stimulates the formation of granulocytes and macrophages from bone marrow progenitor cells. It has various physiological functions, primarily promoting the production, differentiation, activation, and survival of these cells. Additionally, it is a critical homeostatic factor in the lungs, where it is involved in the development and long-term maintenance of alveolar macrophages. The GM-CSF mRNA used in this study is a special type called 5-Methoxy UTP, which has been modified with Cap1 structure and 5-Methoxy UTP to optimize its UTR and poly A regions. This modification significantly improves mRNA translation efficiency and expression levels, while reducing its immunogenicity and cytotoxicity.

Cas9 mRNA

Cas9 mRNA is modified with Cap1 and poly A structures, using N1 methyl pseudo-uridine, to enable expression of Cas9 protein from Streptococcus pyogenes in eukaryotic cells. The N-terminal and C-terminal of the Cas9 protein contain NLS sequences, allowing it to enter the cell nucleus. By binding with sgRNA, the Cas9 protein can target and cleave genomic DNA, creating double-strand breaks that can be repaired through NHEJ or HDR mechanisms for gene editing. The C-terminal of the Cas9 protein is tagged with HA for analysis and detection purposes.

RhCap001(ammonium salt)

RhCap001 is a new type of cap-like object with proprietary intellectual property rights. This product is used for transcription with a starting sequence of 5'AG3'. Compared to the Cap1 and Cap0 generated by traditional capping methods, the EM1003 cap structure allows mRNA to have higher in vivo activity and translation efficiency.

N1-Me-Pseudo-UTP(sodium salt)

N1-Me-Pseudo-UTP is a modified nucleotide used in various molecular biology applications such as in vitro transcription and siRNA synthesis. It can be added to mRNA through in vitro transcription to reduce the mRNA's immunogenicity and improve its expression efficiency by increasing ribosome binding. Experimental evidence has shown that N1-Me-Pseudo-UTP can further enhance mRNA expression efficiency in cells and mice compared to pseudo-UTP, while reducing mRNA's immunogenicity. This product is a sodium salt solution of N1-Me-Pseudo-UTP with a purity of≥ 98% (HPLC), a concentration of 100mM±5mM, and no contamination of endonucleases, exonucleases, and ribonucleases.

Pseudo UTP(sodium salt)

Pseudouridine triphosphate (pUTP) is a commonly used modified nucleotide in molecular biology. It finds various applications, such as in vitro transcription and siRNA synthesis. Studies have shown that mRNA synthesized using pUTP has improved stability, translation efficiency, and reduced immunogenicity, making it widely used in the field of disease research. Our product is a sodium salt solution of pUTP with a purity of≥ 96% (HPLC) and a concentration of 100mM±5mM. It is free from contamination of endonucleases, exonucleases, and ribonucleases.

T7 RNA Polymerase

This product is a recombinant source of T7 RNA polymerase from Escherichia coli. It is a DNA-dependent RNA polymerase that specifically recognizes the T7 promoter and synthesizes RNA chains complementary to the downstream template strand. It uses single-stranded or double-stranded DNA templates containing the T7 promoter sequence and NTPs as substrates.

RNase inhibitor,Murine

This product is a recombinant mouse RNase inhibitor expressed and purified in E. coli. It can bind to RNase A, RNase B, or RNase C in a non-competitive manner in a 1:1 ratio, thereby inhibiting the activity of these three enzymes and protecting RNA from degradation. The inhibitor is compatible with various commercially available Reverse Transcriptase and DNA Polymerase enzymes. Mouse RNase inhibitor has higher antioxidant activity due to the absence of two cysteine residues in human proteins that are highly sensitive to oxidation, making it more suitable for experiments sensitive to high DTT levels, such as qPCR.

RHT19

RHT19 is a novel ionizable cationic lipid that forms lipid nanoparticles (LNPs) in combination with other lipids, used for delivery in mRNA-based research.