Cas9 mRNA
Product Type
mRNA Spot products
Category
Gene Editing mRNA
Art.No
ER3001
Description
Cas9 mRNA is modified with Cap1 and poly A structures, using N1 methyl pseudo-uridine, to enable expression of Cas9 protein from Streptococcus pyogenes in eukaryotic cells. The N-terminal and C-terminal of the Cas9 protein contain NLS sequences, allowing it to enter the cell nucleus. By binding with sgRNA, the Cas9 protein can target and cleave genomic DNA, creating double-strand breaks that can be repaired through NHEJ or HDR mechanisms for gene editing. The C-terminal of the Cas9 protein is tagged with HA for analysis and detection purposes.Product Information
| Product Type | mRNA Spot products |
|---|---|
| Category | Gene Editing mRNA |
| Art.No | ER3001 |
| Scale | ①50μg②200μg③500μg④1mg |
| CDS length/nt | 4245 |
| Purity/% | ≥90 |
| Modified Nucleotide | N1-Me-Pseudo UTP |
| Capping efficiency/% | 不展示 |
| Cap categories | cap1 |
| Storage buffer | RNase-free water |
| Storage temperature | -80℃ |
| Transportation | Dry ice |
Note
This product is only intended for research purposes and should not be used for therapeutic or diagnostic purposes in humans or animals.
Product manual
Cas9 mRNA Product manual
COA
COA of Cas9 mRNA